Journal: Molecular Vision

Article Title: Developmentally dynamic colocalization patterns of DSCAM with adhesion and synaptic proteins in the mouse retina

doi:

Figure Lengend Snippet: Table of colocalization.

Article Snippet: Rabbit anti 14-3-3 Epsilon (ywhae; Cell Signaling Technology, Danvers, MA; 9635; 1:400), Rabbit anti 14-3-3 Eta (ywhah; Cell Signaling Technology; 9640; 1:400), Mouse anti β-Catenin (BD Biosciences San Jose, CA; 610153; 1:200), Goat anti Bassoon (Santa Cruz Biotechnology, Santa Cruz, CA; sc-18565; 1:400), Mouse anti Cadherin 8.1 (DSHB, Iowa City, IA; 1:100), Mouse anti Cask (NeuroMab, Davis, CA; 75-000; 1:400), Mouse anti Connexin 36 (Millipore, Darmstadt, Germany; MAB3045; 1:400), Mouse anti Connexin 45 (Invitrogen, Franklin Lakes, NJ; 415 800; 1:400), Mouse anti CTBP2 (BD Biosciences; 612042; 1:400), Goat anti DSCAM (R&D Systems Minneapolis, MN; AF3315; 1:500 for WBA, 1:100 for IHC), Rabbit anti GABAa receptor delta subunit 2 (Synaptic Systems, Göttingen, Germany; 224 003; 1:400), Mouse anti Gap43 (Millipore; MAB347; 1:400), Mouse anti GluR1 (NeuroMab; 75-327; 1:500), Mouse anti GluR2 (NeuroMab; 75-002; 1:400), Rabbit anti GluR4 (Cell Signaling Technologies; 8070; 1:400), Goat anti GluR5 (Santa Cruz Biotechnology; Sc-7616; 1:100), Mouse anti GlyR (Synaptic Systems, Göttingen, Germany; 146 011; 1:400), Mouse anti ß-protocadherin (NeuroMab; 75-185; 1:400), Rabbit anti Magi2 (Sigma-Aldrich, St. Louis, MO; HPA 013 650; 1:400), Rabbit anti Magi3 (Sigma-Aldrich; HPA 007 923; 1:400), Mouse anti Mitochondria (Ox Phos; Life Technologies; A21348; 1:750), Rat anti N-Catenin (DSHB, IA; 1:200), Mouse anti Neuroligin 1 (NeuroMab; 75-160; 1:400), Rabbit anti NMDA 2AB (Millipore; AB1548; 1:400), Rabbit anti NMDA 2b (NeuroMab; Q00960; 1:400), Mouse anti NR1 (NeuroMab; 75-272; 1:500), Rabbit anti P2X2 (Alomone labs, Jerusalem, Israel; APR-003; 1:100), Rabbit anti P2X7 (Millipore; AB5246; 1:100), Mouse anti PSD95 (NeuroMab; 75-028; 1:200), Rat anti R-Cadherin (MRCD5; DSHB; 1:200), Rabbit anti Rim2 (Synaptic System; 140 103; 1:400), Mouse anti Sap102 (NeuroMab; 75-058; 1:200), Mouse anti Shank (NeuroMab; 75-089; 1:400), Rabbit anti Synapsin (Cell Signaling Technology; 5297; 1:400), Mouse anti synaptotagmin2 (ZIRC, Eugene, OR; 1:500), Mouse anti Syntaxin I (Sigma-Aldrich; S 0664; 1:1000), Rabbit anti Syntaxin II (Synaptic Systems; 110-022; 1:200), Rabbit anti Syntaxin III (Novus Biologicals, Littleton, CO; AB4113; 1:1000), Rabbit anti Syntaxin IV (Millipore; AB5330; 1:200), Rabbit anti ZO-1 (Invitrogen; 61-7300; 1:400), Rabbit anti ZO-2 (Invitrogen; 71-1400; 1:400) and Rabbit anti ZONAB (Invitrogen; 48-2800; 1:400.

Techniques:

Journal: Molecular Vision

Article Title: Developmentally dynamic colocalization patterns of DSCAM with adhesion and synaptic proteins in the mouse retina

doi:

Figure Lengend Snippet: DSCAM colocalizes with catenins in the adult retina. A - C : Sections of retina were stained with antibodies to DSCAM, PSD95 and bassoon. DSCAM staining appeared to overlap minimally with synaptic markers. D : Retinal sections were stained with two different antibodies to DSCAM. Colocalization of markers was apparent in white (inset box). A bar chart readout of the colocalization by laminar depth, running from S1 (top) to S5 (bottom). The degree of protein colocalizing is indicated by the length of bar on the x-axis. Flipped controls were generated by rotating one of the channels 180 degrees across the vertical axis (so as to preserve S1-S5 but provide a randomized control). E : Retinal sections were stained with antibodies to bassoon and GABAa∂2. Colocalization of markers was apparent in white and in the bar chart (inset box). F : Retinal sections were stained with antibodies to GluR2 and bassoon. No colocalization of markers was apparent in white (inset box), and as depicted in the bar chart. G , Retinal sections from adult mice were stained with antibodies to Syntaxin1 and DSCAM. Minimal colocalization of markers was apparent (inset box) and in the bar charts. H : Retinal sections from adult mice were stained with antibodies to Syntaxin3 and DSCAM. No colocalization of markers was apparent in white (inset box) and as depicted in the bar charts. I : Retinal sections from adult mice were stained with antibodies to the adherens junctions marker ß-catenin and DSCAM. Colocalization of markers was apparent in white (inset box) and in the bar charts. J : Retinal sections from adult mice were stained with antibodies to the adherens junctions marker α-n-catenin and DSCAM. Colocalization of markers was apparent in white (inset box) and in the bar charts. K : Retinal sections from adult mice were stained with antibodies to the junction marker Zonula Occludens-1 and DSCAM. No colocalization of markers was apparent in white (inset box) or as depicted by the bar charts. L : Retinal sections from adult mice were stained with antibodies to the gap junction marker Connexin 45 and DSCAM. No colocalization of markers was apparent in white (inset box) or by the bar charts. The scale bar in ( A ) is equivalent to 12.5 µm in A , 18.9 µm in B and C , 53.25 µm in D - L and 7.9 µm in the insets from D - L .

Article Snippet: Rabbit anti 14-3-3 Epsilon (ywhae; Cell Signaling Technology, Danvers, MA; 9635; 1:400), Rabbit anti 14-3-3 Eta (ywhah; Cell Signaling Technology; 9640; 1:400), Mouse anti β-Catenin (BD Biosciences San Jose, CA; 610153; 1:200), Goat anti Bassoon (Santa Cruz Biotechnology, Santa Cruz, CA; sc-18565; 1:400), Mouse anti Cadherin 8.1 (DSHB, Iowa City, IA; 1:100), Mouse anti Cask (NeuroMab, Davis, CA; 75-000; 1:400), Mouse anti Connexin 36 (Millipore, Darmstadt, Germany; MAB3045; 1:400), Mouse anti Connexin 45 (Invitrogen, Franklin Lakes, NJ; 415 800; 1:400), Mouse anti CTBP2 (BD Biosciences; 612042; 1:400), Goat anti DSCAM (R&D Systems Minneapolis, MN; AF3315; 1:500 for WBA, 1:100 for IHC), Rabbit anti GABAa receptor delta subunit 2 (Synaptic Systems, Göttingen, Germany; 224 003; 1:400), Mouse anti Gap43 (Millipore; MAB347; 1:400), Mouse anti GluR1 (NeuroMab; 75-327; 1:500), Mouse anti GluR2 (NeuroMab; 75-002; 1:400), Rabbit anti GluR4 (Cell Signaling Technologies; 8070; 1:400), Goat anti GluR5 (Santa Cruz Biotechnology; Sc-7616; 1:100), Mouse anti GlyR (Synaptic Systems, Göttingen, Germany; 146 011; 1:400), Mouse anti ß-protocadherin (NeuroMab; 75-185; 1:400), Rabbit anti Magi2 (Sigma-Aldrich, St. Louis, MO; HPA 013 650; 1:400), Rabbit anti Magi3 (Sigma-Aldrich; HPA 007 923; 1:400), Mouse anti Mitochondria (Ox Phos; Life Technologies; A21348; 1:750), Rat anti N-Catenin (DSHB, IA; 1:200), Mouse anti Neuroligin 1 (NeuroMab; 75-160; 1:400), Rabbit anti NMDA 2AB (Millipore; AB1548; 1:400), Rabbit anti NMDA 2b (NeuroMab; Q00960; 1:400), Mouse anti NR1 (NeuroMab; 75-272; 1:500), Rabbit anti P2X2 (Alomone labs, Jerusalem, Israel; APR-003; 1:100), Rabbit anti P2X7 (Millipore; AB5246; 1:100), Mouse anti PSD95 (NeuroMab; 75-028; 1:200), Rat anti R-Cadherin (MRCD5; DSHB; 1:200), Rabbit anti Rim2 (Synaptic System; 140 103; 1:400), Mouse anti Sap102 (NeuroMab; 75-058; 1:200), Mouse anti Shank (NeuroMab; 75-089; 1:400), Rabbit anti Synapsin (Cell Signaling Technology; 5297; 1:400), Mouse anti synaptotagmin2 (ZIRC, Eugene, OR; 1:500), Mouse anti Syntaxin I (Sigma-Aldrich; S 0664; 1:1000), Rabbit anti Syntaxin II (Synaptic Systems; 110-022; 1:200), Rabbit anti Syntaxin III (Novus Biologicals, Littleton, CO; AB4113; 1:1000), Rabbit anti Syntaxin IV (Millipore; AB5330; 1:200), Rabbit anti ZO-1 (Invitrogen; 61-7300; 1:400), Rabbit anti ZO-2 (Invitrogen; 71-1400; 1:400) and Rabbit anti ZONAB (Invitrogen; 48-2800; 1:400.

Techniques: Staining, Generated, Marker

Journal: Molecular Vision

Article Title: Developmentally dynamic colocalization patterns of DSCAM with adhesion and synaptic proteins in the mouse retina

doi:

Figure Lengend Snippet: Synaptic and adhesion molecules in the wild-type and Dscam mutant retina. Wild-type and Dscam mutant retina sections were stained with antibodies to synaptic or junction molecules. A and B : Section of wild-type and Dscam mutant retina were stained with antibodies to CTBP2. C and D : Sections of wild-type and Dscam mutant retina were stained with antibodies to PSD95. E and F : Sections of wild-type and Dscam mutant retina were stained with antibodies to Bassoon. G and H : Sections of wild-type and Dscam mutant retina were stained with antibodies to α-n-catenin. I and J : Sections of wild-type and Dscam mutant retina were stained with antibodies to GABAaδ2. K and L : Sections of wild-type and Dscam mutant retina were stained with antibodies to γ-protocadherin. A similar distribution of synaptic markers, considering the disorganization of the Dscam mutant retina, was observed in the genotypes. Scale = 53.25 μm.

Article Snippet: Rabbit anti 14-3-3 Epsilon (ywhae; Cell Signaling Technology, Danvers, MA; 9635; 1:400), Rabbit anti 14-3-3 Eta (ywhah; Cell Signaling Technology; 9640; 1:400), Mouse anti β-Catenin (BD Biosciences San Jose, CA; 610153; 1:200), Goat anti Bassoon (Santa Cruz Biotechnology, Santa Cruz, CA; sc-18565; 1:400), Mouse anti Cadherin 8.1 (DSHB, Iowa City, IA; 1:100), Mouse anti Cask (NeuroMab, Davis, CA; 75-000; 1:400), Mouse anti Connexin 36 (Millipore, Darmstadt, Germany; MAB3045; 1:400), Mouse anti Connexin 45 (Invitrogen, Franklin Lakes, NJ; 415 800; 1:400), Mouse anti CTBP2 (BD Biosciences; 612042; 1:400), Goat anti DSCAM (R&D Systems Minneapolis, MN; AF3315; 1:500 for WBA, 1:100 for IHC), Rabbit anti GABAa receptor delta subunit 2 (Synaptic Systems, Göttingen, Germany; 224 003; 1:400), Mouse anti Gap43 (Millipore; MAB347; 1:400), Mouse anti GluR1 (NeuroMab; 75-327; 1:500), Mouse anti GluR2 (NeuroMab; 75-002; 1:400), Rabbit anti GluR4 (Cell Signaling Technologies; 8070; 1:400), Goat anti GluR5 (Santa Cruz Biotechnology; Sc-7616; 1:100), Mouse anti GlyR (Synaptic Systems, Göttingen, Germany; 146 011; 1:400), Mouse anti ß-protocadherin (NeuroMab; 75-185; 1:400), Rabbit anti Magi2 (Sigma-Aldrich, St. Louis, MO; HPA 013 650; 1:400), Rabbit anti Magi3 (Sigma-Aldrich; HPA 007 923; 1:400), Mouse anti Mitochondria (Ox Phos; Life Technologies; A21348; 1:750), Rat anti N-Catenin (DSHB, IA; 1:200), Mouse anti Neuroligin 1 (NeuroMab; 75-160; 1:400), Rabbit anti NMDA 2AB (Millipore; AB1548; 1:400), Rabbit anti NMDA 2b (NeuroMab; Q00960; 1:400), Mouse anti NR1 (NeuroMab; 75-272; 1:500), Rabbit anti P2X2 (Alomone labs, Jerusalem, Israel; APR-003; 1:100), Rabbit anti P2X7 (Millipore; AB5246; 1:100), Mouse anti PSD95 (NeuroMab; 75-028; 1:200), Rat anti R-Cadherin (MRCD5; DSHB; 1:200), Rabbit anti Rim2 (Synaptic System; 140 103; 1:400), Mouse anti Sap102 (NeuroMab; 75-058; 1:200), Mouse anti Shank (NeuroMab; 75-089; 1:400), Rabbit anti Synapsin (Cell Signaling Technology; 5297; 1:400), Mouse anti synaptotagmin2 (ZIRC, Eugene, OR; 1:500), Mouse anti Syntaxin I (Sigma-Aldrich; S 0664; 1:1000), Rabbit anti Syntaxin II (Synaptic Systems; 110-022; 1:200), Rabbit anti Syntaxin III (Novus Biologicals, Littleton, CO; AB4113; 1:1000), Rabbit anti Syntaxin IV (Millipore; AB5330; 1:200), Rabbit anti ZO-1 (Invitrogen; 61-7300; 1:400), Rabbit anti ZO-2 (Invitrogen; 71-1400; 1:400) and Rabbit anti ZONAB (Invitrogen; 48-2800; 1:400.

Techniques: Mutagenesis, Staining